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replacing water in the tissue with alcohol to a moisture level of 5% and then using a vacuum process to remove the alcohol from the tissue.
8%
76/905
maintaining maximum cellular viability of fresh tissue without long-term storage.
7%
60/905
using chemicals to remove cellular water and controlled rate freezing to prevent ice crystal formation.
57%
514/905
freezing the graft twice and packaging the tissue without solution at minus 80 degrees C.
19%
170/905
freezing the graft in water without an antibiotic solution soak during quarantine, with final storage in liquid nitrogen.
74/905
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Cryopreservation uses chemicals to remove cellular water and controlled rate freezing to prevent ice crystal formation. The tissue is procured, cooled to wet ice temperature for quarantine, and then stored in a container with cryoprotectant solution of dimethyl sulfoxide or glycerol which displaces the cellular water. The controlled rate freezing is then done to prevent ice crystal formation. Fresh allografts are not frozen in order to maintain maximum cellular viability, and this process limits the shelf life of osteochondral allografts. Freeze-drying involves replacement of water in the tissue with alcohol to a moisture level of 5% and then uses a vacuum process to remove the alcohol from the tissue. Preparation of fresh frozen grafts involves freezing the graft twice and packaging the tissue without solution at minus 80 degrees C.
2.8
(18)
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